View source for Bulk RNA-sequencing (whole transcriptome)

* [[Page authors|Page authors]]: [[Michiel Perneel]], [[PRIMO]]
* [[Responsible curator|Responsible curator]]:  [[User:Kate Evans|Kate Evans]]
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High-throughput sequencing of RNA transcribed by an individual or population of a given organism, or a community of species. 
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| '''Approach:''' filtering, RNA extraction, sequencing, bioinformatics
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| '''Context:''' ''in situ'', culturing, incubations
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| '''Spatial scale:''' L
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| '''Temporal scale:''' seconds, days, seasons
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| '''Units:''' gene expression, e.g. TPM
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| '''Community captured:''' size-fractioned, e.g. 0.2 - 250 µm, cultured or target species
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| '''Co-measurements:''' Other measurements required for interpretation of results e.g., temperature, salinity, nutrients, physiological data
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== Method Overview ==

== Output ==

=== Scale of measurement ===

=== Data generated ===

=== Units & currency ===

=== Sample size ===

=== Repositories & databases ===

== Limitations ==

== Example Applications & Protocols ==

=== Classic examples ===

=== Recent applications ===

=== Common calculations/conversions ===
* Normalised expression, for example transcripts per million (TPM)
* Quantitative estimates, for example transcripts per L (TPL)

== References ==

[[Category:Main Pages|Model types]]