View source for Enzyme assay/alkaline phosphatase with fluorescently labeled substrate

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* [[Page authors|Page authors]]: [[PRIMO]]
* [[Responsible curator|Responsible curator]]:  [[User:Hagi BucknWise|Hagen Buck-Wiese]]
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! Alkaline phosphatase
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| '''Approach:''' enzyme assay
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| '''Context:''' incubation, lab
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| '''Spatial scale:''' low
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| '''Temporal scale:''' low; hours to days
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| '''Units:''' mol fl tag L-1 h-1
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| '''Community captured:''' microalgae and heterotrophs
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| '''Co-measurements:''' cell abundance
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== Method Overview ==

This enzyme assay measures alkaline phosphatase by time course incubations with fluorescently labeled substrate.

The assay is performed in continous mode (flow through fluorimeter) mode on bulk, size-fractionated, or single cell samples.

=== Scale of measurement ===

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=== Data generated ===

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=== Units & currency ===

Units are mol fl tag L-1 h-1. The currency is P.

=== Sample size ===

Typical samples are < 1 L in volume.

=== Repositories & databases ===

== Limitations ==

== Example Applications & Protocols ==

=== Classic examples ===
* 

=== Recent applications ===
* Su et al. (2023) ''A dataset of global ocean alkaline phosphatase activity'' <ref name="Su2023">Su et al. (2023). A dataset of global ocean alkaline phosphatase activity. ''Scientific Data'', 10(1). https://doi.org/10.1038/s41597-023-02081-7</ref>
* Thomson et al. (2020) ''Relative Importance of Phosphodiesterase vs. Phosphomonoesterase (Alkaline Phosphatase) Activities for Dissolved Organic Phosphorus Hydrolysis in Epi- and Mesopelagic Waters'' <ref name="Thomson2020">Thomson et al. (2020). Relative Importance of Phosphodiesterase vs. Phosphomonoesterase (Alkaline Phosphatase) Activities for Dissolved Organic Phosphorus Hydrolysis in Epi- and Mesopelagic Waters. ''Frontiers in Earth Science'', 8. https://doi.org/10.3389/feart.2020.560893</ref>

=== Common calculations/conversions ===
* 

== References ==

[[Category:Main Pages|Model types]]