View source for Enzyme assay with DNS

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* [[Page authors|Page authors]]: [[PRIMO]]
* [[Responsible curator|Responsible curator]]:  [[User:Hagi BucknWise|Hagen Buck-Wiese]]
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{| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;"
! Polysaccharide hydrolysis
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| '''Approach:''' enzyme assay
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| '''Context:''' incubation, lab
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| '''Spatial scale:''' low
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| '''Temporal scale:''' low; hours to days
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| '''Units:''' mol mL-1 substrate h-1
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| '''Community captured:''' heterotrophs
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| '''Co-measurements:''' cell abundance
|}
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== Method Overview ==

This enzyme assay measures polysaccharide hydrolysis by time course incubation with DNS (3,5-dinitrosalicylic acid) <ref name="Kim2014">Kim et al. (2014). Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application. ''Journal of Microbiology and Biotechnology'', 24(11), 1559-1565. https://doi.org/10.4014/jmb.1405.05052</ref>.

The assay is performed on bulk samples.

=== Scale of measurement ===

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=== Data generated ===

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=== Units & currency ===

Units are mol mL-1 substrate h-1. The currency is carbon.

=== Sample size ===

Typical samples are < 1 L in volume.

=== Repositories & databases ===

== Limitations ==

Reports potential rates (Vmax) and bottle effects can occur during incubation period.

== Example Applications & Protocols ==

=== Classic examples ===
* Kim et al. (2014) ''Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application'' <ref name="Kim2014" />

=== Recent applications ===
* 

=== Common calculations/conversions ===
* 

== References ==

[[Category:Main Pages|Model types]]