https://biogeoscapes.net//wiki/index.php?action=history&feed=atom&title=Enzyme_assay%2Falkaline_phosphatase_with_fluorescently_labeled_substrate Enzyme assay/alkaline phosphatase with fluorescently labeled substrate - Revision history 2026-05-27T22:39:14Z Revision history for this page on the wiki MediaWiki 1.45.0 https://biogeoscapes.net//wiki/index.php?title=Enzyme_assay/alkaline_phosphatase_with_fluorescently_labeled_substrate&diff=642&oldid=prev Hagi BucknWise: Created page with "{{BreadcrumbsSecondaryProduction}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Alkaline phosphatase |- | '''Approach:''' enzyme assay |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' low |- | '''Temporal scale:''' low; hours to days |- | '''Units:''' mol f..." 2026-05-11T18:41:42Z <p>Created page with &quot;{{BreadcrumbsSecondaryProduction}} * <a href="/wiki/index.php?title=Page_authors" title="Page authors">Page authors</a>: <a href="/wiki/index.php?title=PRIMO&amp;action=edit&amp;redlink=1" class="new" title="PRIMO (page does not exist)">PRIMO</a> * <a href="/wiki/index.php?title=Responsible_curator" title="Responsible curator">Responsible curator</a>: <a href="/wiki/index.php?title=User:Hagi_BucknWise" title="User:Hagi BucknWise">Hagen Buck-Wiese</a> ---- __TOC__ &lt;div class=&quot;model-box&quot;&gt; {| class=&quot;model-ib&quot; style=&quot;float:right; margin-left:1em; margin-bottom:1em;&quot; ! Alkaline phosphatase |- | &#039;&#039;&#039;Approach:&#039;&#039;&#039; enzyme assay |- | &#039;&#039;&#039;Context:&#039;&#039;&#039; incubation, lab |- | &#039;&#039;&#039;Spatial scale:&#039;&#039;&#039; low |- | &#039;&#039;&#039;Temporal scale:&#039;&#039;&#039; low; hours to days |- | &#039;&#039;&#039;Units:&#039;&#039;&#039; mol f...&quot;</p> <p><b>New page</b></p><div>{{BreadcrumbsSecondaryProduction}}<br /> <br /> * [[Page authors|Page authors]]: [[PRIMO]]<br /> * [[Responsible curator|Responsible curator]]: [[User:Hagi BucknWise|Hagen Buck-Wiese]]<br /> ----<br /> <br /> __TOC__<br /> &lt;div class=&quot;model-box&quot;&gt;<br /> {| class=&quot;model-ib&quot; style=&quot;float:right; margin-left:1em; margin-bottom:1em;&quot;<br /> ! Alkaline phosphatase<br /> |-<br /> | &#039;&#039;&#039;Approach:&#039;&#039;&#039; enzyme assay<br /> |-<br /> | &#039;&#039;&#039;Context:&#039;&#039;&#039; incubation, lab<br /> |-<br /> | &#039;&#039;&#039;Spatial scale:&#039;&#039;&#039; low<br /> |-<br /> | &#039;&#039;&#039;Temporal scale:&#039;&#039;&#039; low; hours to days<br /> |-<br /> | &#039;&#039;&#039;Units:&#039;&#039;&#039; mol fl tag L-1 h-1<br /> |-<br /> | &#039;&#039;&#039;Community captured:&#039;&#039;&#039; microalgae and heterotrophs<br /> |-<br /> | &#039;&#039;&#039;Co-measurements:&#039;&#039;&#039; cell abundance<br /> |}<br /> &lt;/div&gt;<br /> &lt;div style=&quot;clear:both&quot;&gt;&lt;/div&gt;<br /> <br /> == Method Overview ==<br /> <br /> This enzyme assay measures alkaline phosphatase by time course incubations with fluorescently labeled substrate.<br /> <br /> The assay is performed in continous mode (flow through fluorimeter) mode on bulk, size-fractionated, or single cell samples.<br /> <br /> === Scale of measurement ===<br /> <br /> &lt;!-- TODO: describe spatial and temporal scale of this method --&gt;<br /> <br /> === Data generated ===<br /> <br /> &lt;!-- TODO: describe what data this method generates and what it represents --&gt;<br /> <br /> === Units &amp; currency ===<br /> <br /> Units are mol fl tag L-1 h-1. The currency is P.<br /> <br /> === Sample size ===<br /> <br /> Typical samples are &lt; 1 L in volume.<br /> <br /> === Repositories &amp; databases ===<br /> <br /> == Limitations ==<br /> <br /> == Example Applications &amp; Protocols ==<br /> <br /> === Classic examples ===<br /> * <br /> <br /> === Recent applications ===<br /> * Su et al. (2023) &#039;&#039;A dataset of global ocean alkaline phosphatase activity&#039;&#039; &lt;ref name=&quot;Su2023&quot;&gt;Su et al. (2023). A dataset of global ocean alkaline phosphatase activity. &#039;&#039;Scientific Data&#039;&#039;, 10(1). https://doi.org/10.1038/s41597-023-02081-7&lt;/ref&gt;<br /> * Thomson et al. (2020) &#039;&#039;Relative Importance of Phosphodiesterase vs. Phosphomonoesterase (Alkaline Phosphatase) Activities for Dissolved Organic Phosphorus Hydrolysis in Epi- and Mesopelagic Waters&#039;&#039; &lt;ref name=&quot;Thomson2020&quot;&gt;Thomson et al. (2020). Relative Importance of Phosphodiesterase vs. Phosphomonoesterase (Alkaline Phosphatase) Activities for Dissolved Organic Phosphorus Hydrolysis in Epi- and Mesopelagic Waters. &#039;&#039;Frontiers in Earth Science&#039;&#039;, 8. https://doi.org/10.3389/feart.2020.560893&lt;/ref&gt;<br /> <br /> === Common calculations/conversions ===<br /> * <br /> <br /> == References ==<br /> <br /> [[Category:Main Pages|Model types]]</div> Hagi BucknWise