Enzyme assay with DNS - Revision history

Hagi BucknWise at 18:39, 11 May 2026

2026-05-11T18:39:50Z

← Older revision		Revision as of 11:39, 11 May 2026
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	== Method Overview ==		== Method Overview ==

	This enzyme assay measures polysaccharide hydrolysis by time course incubation with DNS (2,4-~~dinitrophenylsulfonyl-group~~) <ref name="Kim2014">Kim et al. (2014). Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application. ''Journal of Microbiology and Biotechnology'', 24(11), 1559-1565. https://doi.org/10.4014/jmb.1405.05052</ref>.		This enzyme assay measures polysaccharide hydrolysis by time course incubation with DNS (3,5-dinitrosalicylic acid) <ref name="Kim2014">Kim et al. (2014). Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application. ''Journal of Microbiology and Biotechnology'', 24(11), 1559-1565. https://doi.org/10.4014/jmb.1405.05052</ref>.

	The assay is performed on bulk samples.		The assay is performed on bulk samples.

Hagi BucknWise at 18:38, 11 May 2026

2026-05-11T18:38:58Z

← Older revision		Revision as of 11:38, 11 May 2026
Line 29:		Line 29:
	== Method Overview ==		== Method Overview ==

	This enzyme assay measures polysaccharide hydrolysis by time course ~~incuabtion~~ with DNS<ref name="Kim2014">Kim et al. (2014). Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application. ''Journal of Microbiology and Biotechnology'', 24(11), 1559-1565. https://doi.org/10.4014/jmb.1405.05052</ref>.		This enzyme assay measures polysaccharide hydrolysis by time course incubation with DNS (2,4-dinitrophenylsulfonyl-group) <ref name="Kim2014">Kim et al. (2014). Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application. ''Journal of Microbiology and Biotechnology'', 24(11), 1559-1565. https://doi.org/10.4014/jmb.1405.05052</ref>.

	The assay is performed on bulk samples.		The assay is performed on bulk samples.

Hagi BucknWise: Created page with "{{BreadcrumbsSecondaryProduction}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- TOC
{| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Polysaccharide hydrolysis |- | '''Approach:''' enzyme assay |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' low |- | '''Temporal scale:''' low; hours to days |- | '''Units:'''..."

2026-05-11T18:35:19Z

Created page with "{{BreadcrumbsSecondaryProduction}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Polysaccharide hydrolysis |- | '''Approach:''' enzyme assay |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' low |- | '''Temporal scale:''' low; hours to days |- | '''Units:'''..."

New page

{{BreadcrumbsSecondaryProduction}}

* [[Page authors|Page authors]]: [[PRIMO]]
* [[Responsible curator|Responsible curator]]: [[User:Hagi BucknWise|Hagen Buck-Wiese]]
----

__TOC__
<div class="model-box">
{| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;"
! Polysaccharide hydrolysis
|-
| '''Approach:''' enzyme assay
|-
| '''Context:''' incubation, lab
|-
| '''Spatial scale:''' low
|-
| '''Temporal scale:''' low; hours to days
|-
| '''Units:''' mol mL-1 substrate h-1
|-
| '''Community captured:''' heterotrophs
|-
| '''Co-measurements:''' cell abundance
|}
</div>
<div style="clear:both"></div>

== Method Overview ==

This enzyme assay measures polysaccharide hydrolysis by time course incuabtion with DNS<ref name="Kim2014">Kim et al. (2014). Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application. ''Journal of Microbiology and Biotechnology'', 24(11), 1559-1565. https://doi.org/10.4014/jmb.1405.05052</ref>.

The assay is performed on bulk samples.

=== Scale of measurement ===



=== Data generated ===



=== Units & currency ===

Units are mol mL-1 substrate h-1. The currency is carbon.

=== Sample size ===

Typical samples are < 1 L in volume.

=== Repositories & databases ===

== Limitations ==

Reports potential rates (Vmax) and bottle effects can occur during incubation period.

== Example Applications & Protocols ==

=== Classic examples ===
* Kim et al. (2014) ''Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application'' <ref name="Kim2014" />

=== Recent applications ===
*

=== Common calculations/conversions ===
*

== References ==

[[Category:Main Pages|Model types]]