Enzyme assay with RBB labeled polysaccharides - Revision history

Hagi BucknWise at 18:33, 11 May 2026

2026-05-11T18:33:24Z

← Older revision		Revision as of 11:33, 11 May 2026
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	== Limitations ==		== Limitations ==

	rate measurements ~~at max~~ (Vmax). ~~bottle~~ effects~~, potential rates~~.		These rate measurements report potential rates (Vmax). Bottle effects can occur due to the length of the required incubation period.

	== Example Applications & Protocols ==		== Example Applications & Protocols ==

Hagi BucknWise at 18:31, 11 May 2026

2026-05-11T18:31:50Z

← Older revision		Revision as of 11:31, 11 May 2026
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	This enzyme assay measures polysaccharide hydrolysis by time course incubation with RBB labeled polysaccharides<ref name="Wirth1992">Wirth & Wolf (1992). Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons. ''Soil Biology and Biochemistry'', 24(6), 511-519. https://doi.org/10.1016/0038-0717(92)90074-8</ref>.		This enzyme assay measures polysaccharide hydrolysis by time course incubation with RBB labeled polysaccharides<ref name="Wirth1992">Wirth & Wolf (1992). Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons. ''Soil Biology and Biochemistry'', 24(6), 511-519. https://doi.org/10.1016/0038-0717(92)90074-8</ref>.

	~~Applicable variants include:~~ bulk.		The assay is performed in discrete mode on bulk samples.

	=== Scale of measurement ===		=== Scale of measurement ===

	~~Spatial resolution is low (horizontal)~~ and ~~low (vertical). Temporal resolution is low, with a time integration~~ of ~~hours to days.~~		<!-- TODO: describe spatial and temporal scale of this method -->

	=== Data generated ===		=== Data generated ===

	~~Yields rates in units of relative OD units.~~		<!-- TODO: describe what data this method generates and what it represents -->

	=== Units & currency ===		=== Units & currency ===
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	=== Sample size ===		=== Sample size ===

	< L.		Typical samples are < 1 L in volume.

	=== Repositories & databases ===		=== Repositories & databases ===

Hagi BucknWise at 18:06, 11 May 2026

2026-05-11T18:06:57Z

← Older revision		Revision as of 11:06, 11 May 2026
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	== Method Overview ==		== Method Overview ==

	~~Time~~ course incubation with RBB labeled polysaccharides<ref name="~~Wirth and Wolf~~">Wirth & Wolf (1992). Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons. ''Soil Biology and Biochemistry'', 24(6), 511-519. https://doi.org/10.1016/0038-0717(92)90074-8</ref>.		This enzyme assay measures polysaccharide hydrolysis by time course incubation with RBB labeled polysaccharides<ref name="Wirth1992">Wirth & Wolf (1992). Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons. ''Soil Biology and Biochemistry'', 24(6), 511-519. https://doi.org/10.1016/0038-0717(92)90074-8</ref>.

	Applicable variants include: bulk.		Applicable variants include: bulk.
Line 58:		Line 58:

	=== Classic examples ===		=== Classic examples ===
	* Wirth & Wolf (1992) ''Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons'' <ref name="~~Wirth and Wolf~~" />		* Wirth & Wolf (1992) ''Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons'' <ref name="Wirth1992" />

	=== Recent applications ===		=== Recent applications ===
	* Zohary et al. (2000) ''The role of nutrients in decomposition of a thecate dinoflagellate'' <ref name="~~Zohary et al~~">Zohary et al. (2000). The role of nutrients in decomposition of a thecate dinoflagellate. ''Limnology and Oceanography'', 45(1), 123-130. https://doi.org/10.4319/lo.2000.45.1.0123</ref>		* Zohary et al. (2000) ''The role of nutrients in decomposition of a thecate dinoflagellate'' <ref name="Zohary2000">Zohary et al. (2000). The role of nutrients in decomposition of a thecate dinoflagellate. ''Limnology and Oceanography'', 45(1), 123-130. https://doi.org/10.4319/lo.2000.45.1.0123</ref>

	=== Common calculations/conversions ===		=== Common calculations/conversions ===

Hagi BucknWise at 17:53, 11 May 2026

2026-05-11T17:53:20Z

← Older revision		Revision as of 10:53, 11 May 2026
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	== Method Overview ==		== Method Overview ==

	Time course incubation with RBB labeled polysaccharides<ref name="~~0038071792900748~~">Wirth & Wolf (1992). Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons. ''Soil Biology and Biochemistry'', 24(6), 511-519. https://doi.org/10.1016/0038-0717(92)90074-8</ref>.		Time course incubation with RBB labeled polysaccharides<ref name="Wirth and Wolf">Wirth & Wolf (1992). Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons. ''Soil Biology and Biochemistry'', 24(6), 511-519. https://doi.org/10.1016/0038-0717(92)90074-8</ref>.

	Applicable variants include: bulk.		Applicable variants include: bulk.
Line 58:		Line 58:

	=== Classic examples ===		=== Classic examples ===
	* Wirth & Wolf (1992) ''Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons'' <ref name="~~0038071792900748~~" />		* Wirth & Wolf (1992) ''Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons'' <ref name="Wirth and Wolf" />

	=== Recent applications ===		=== Recent applications ===
	* Zohary et al. (2000) ''The role of nutrients in decomposition of a thecate dinoflagellate'' <ref name="~~lo20004510123~~">Zohary et al. (2000). The role of nutrients in decomposition of a thecate dinoflagellate. ''Limnology and Oceanography'', 45(1), 123-130. https://doi.org/10.4319/lo.2000.45.1.0123</ref>		* Zohary et al. (2000) ''The role of nutrients in decomposition of a thecate dinoflagellate'' <ref name="Zohary et al">Zohary et al. (2000). The role of nutrients in decomposition of a thecate dinoflagellate. ''Limnology and Oceanography'', 45(1), 123-130. https://doi.org/10.4319/lo.2000.45.1.0123</ref>

	=== Common calculations/conversions ===		=== Common calculations/conversions ===

Hagi BucknWise: Created page with "{{BreadcrumbsSecondaryProduction}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- TOC
{| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Polysaccharide hydrolysis |- | '''Approach:''' enzyme assay |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' low |- | '''Temporal scale:''' low; hours to days |- | '''Units:'''..."

2026-05-11T17:52:12Z

Created page with "{{BreadcrumbsSecondaryProduction}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Polysaccharide hydrolysis |- | '''Approach:''' enzyme assay |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' low |- | '''Temporal scale:''' low; hours to days |- | '''Units:'''..."

New page

{{BreadcrumbsSecondaryProduction}}

* [[Page authors|Page authors]]: [[PRIMO]]
* [[Responsible curator|Responsible curator]]: [[User:Hagi BucknWise|Hagen Buck-Wiese]]
----

__TOC__
<div class="model-box">
{| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;"
! Polysaccharide hydrolysis
|-
| '''Approach:''' enzyme assay
|-
| '''Context:''' incubation, lab
|-
| '''Spatial scale:''' low
|-
| '''Temporal scale:''' low; hours to days
|-
| '''Units:''' relative OD units
|-
| '''Community captured:''' heterotrophs
|-
| '''Co-measurements:''' cell abundance
|}
</div>
<div style="clear:both"></div>

== Method Overview ==

Time course incubation with RBB labeled polysaccharides<ref name="0038071792900748">Wirth & Wolf (1992). Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons. ''Soil Biology and Biochemistry'', 24(6), 511-519. https://doi.org/10.1016/0038-0717(92)90074-8</ref>.

Applicable variants include: bulk.

=== Scale of measurement ===

Spatial resolution is low (horizontal) and low (vertical). Temporal resolution is low, with a time integration of hours to days.

=== Data generated ===

Yields rates in units of relative OD units.

=== Units & currency ===

Units are relative OD units. The currency is carbon.

=== Sample size ===

< L.

=== Repositories & databases ===

== Limitations ==

rate measurements at max (Vmax). bottle effects, potential rates.

== Example Applications & Protocols ==

=== Classic examples ===
* Wirth & Wolf (1992) ''Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons'' <ref name="0038071792900748" />

=== Recent applications ===
* Zohary et al. (2000) ''The role of nutrients in decomposition of a thecate dinoflagellate'' <ref name="lo20004510123">Zohary et al. (2000). The role of nutrients in decomposition of a thecate dinoflagellate. ''Limnology and Oceanography'', 45(1), 123-130. https://doi.org/10.4319/lo.2000.45.1.0123</ref>

=== Common calculations/conversions ===
*

== References ==

[[Category:Main Pages|Model types]]