Winkler light-dark dissolved O2 bottle - Revision history

Anabelvonjackowski: /* Method Overview */

2026-05-15T17:57:29Z

Method Overview

← Older revision		Revision as of 10:57, 15 May 2026
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	Dissolved O₂ measures the gas concentrations of oxygen from the depths of interest. This measurement can be measured using the Winkler method via iodometric titration because dissolved oxygen does not directly oxidize iodide to iodine and a multi-step reaction in which manganese acts as an intermediate “transfer” agent. <ref name = "Winkler1888">Winkler, L. W. (1888). Die Bestimmung des im Wasser gelösten Sauerstoffes. Berichte der Deutschen Chemischen Gesellschaft, 21(2), 2843–2854. https://doi.org/10.1002/cber.188802102122</ref>.		Dissolved O₂ measures the gas concentrations of oxygen from the depths of interest. This measurement can be measured using the Winkler method via iodometric titration because dissolved oxygen does not directly oxidize iodide to iodine and a multi-step reaction in which manganese acts as an intermediate “transfer” agent. <ref name = "Winkler1888">Winkler, L. W. (1888). Die Bestimmung des im Wasser gelösten Sauerstoffes. Berichte der Deutschen Chemischen Gesellschaft, 21(2), 2843–2854. https://doi.org/10.1002/cber.188802102122</ref>.

	The Baltic Marine Environment Protection Commission – Helsinki Commission (HELCOM) has published a sampling guideline see here https://helcom.fi/wp-content/uploads/2019/08/Guidelines-for-sampling-and-determination-of-dissolved-oxygen.pdf		The Baltic Marine Environment Protection Commission – Helsinki Commission (HELCOM) has published a sampling guideline see here: https://helcom.fi/wp-content/uploads/2019/08/Guidelines-for-sampling-and-determination-of-dissolved-oxygen.pdf

	== Step-by-Step Protocol ==		== Step-by-Step Protocol ==

Anabelvonjackowski: /* Method Overview */

2026-05-15T17:57:13Z

Method Overview

← Older revision		Revision as of 10:57, 15 May 2026
Line 46:		Line 46:
	Dissolved O₂ measures the gas concentrations of oxygen from the depths of interest. This measurement can be measured using the Winkler method via iodometric titration because dissolved oxygen does not directly oxidize iodide to iodine and a multi-step reaction in which manganese acts as an intermediate “transfer” agent. <ref name = "Winkler1888">Winkler, L. W. (1888). Die Bestimmung des im Wasser gelösten Sauerstoffes. Berichte der Deutschen Chemischen Gesellschaft, 21(2), 2843–2854. https://doi.org/10.1002/cber.188802102122</ref>.		Dissolved O₂ measures the gas concentrations of oxygen from the depths of interest. This measurement can be measured using the Winkler method via iodometric titration because dissolved oxygen does not directly oxidize iodide to iodine and a multi-step reaction in which manganese acts as an intermediate “transfer” agent. <ref name = "Winkler1888">Winkler, L. W. (1888). Die Bestimmung des im Wasser gelösten Sauerstoffes. Berichte der Deutschen Chemischen Gesellschaft, 21(2), 2843–2854. https://doi.org/10.1002/cber.188802102122</ref>.

			The Baltic Marine Environment Protection Commission – Helsinki Commission (HELCOM) has published a sampling guideline see here https://helcom.fi/wp-content/uploads/2019/08/Guidelines-for-sampling-and-determination-of-dissolved-oxygen.pdf

	== Step-by-Step Protocol ==		== Step-by-Step Protocol ==

Anabelvonjackowski: /* References */

2026-05-15T17:55:39Z

References

← Older revision		Revision as of 10:55, 15 May 2026
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	<ref name="Robinson2008">Robinson, C. (2008). Heterotrophic Bacterial Respiration. In: Kirchman, D. L. (Ed.), ''Microbial Ecology of the Oceans''. Wiley-Blackwell, New Jersey, pp. 299–334.</ref>		<ref name="Robinson2008">Robinson, C. (2008). Heterotrophic Bacterial Respiration. In: Kirchman, D. L. (Ed.), ''Microbial Ecology of the Oceans''. Wiley-Blackwell, New Jersey, pp. 299–334.</ref>

	<ref name="Robinson2005">Robinson, C., & Williams, P. J. l. B. (2005). Respiration and its measurement in surface marine waters. In: del Giorgio, P. A., & Williams, P. J. l. B. (Eds.), ''Respiration in Aquatic Ecosystems''. Oxford University Press, Oxford, pp. 147–180~~. https://doi.org~~</ref>		<ref name="Robinson2005">Robinson, C., & Williams, P. J. l. B. (2005). Respiration and its measurement in surface marine waters. In: del Giorgio, P. A., & Williams, P. J. l. B. (Eds.), ''Respiration in Aquatic Ecosystems''. Oxford University Press, Oxford, pp. 147–180 </ref>

	<ref name="Robinson2026">Robinson, C., Seguro, I., Dall'Olmo, G., Moncoiffe, G., Aranguren-Gassis, M., Aristegui, J., Azzaro, M., Baek, Y. J., Baltar, F., Cohn, M. R., Eissler, Y., Evans, C., Fennel, K., Fernandez-Urruzola, I., Ferron, S., Fukuda, H., Garcia-Martin, E. E., Gifford, S., Goddard-Dwyer, M., Hernandez-Hernandez, N., Herndl, Y., Hyun, J. H., Kim, B., Kirchman, D., Kitidis, Vassilis., LaBrie, R., Lefevre, D., Lonborg, C., Maranger, R., Martinez-Garcia, S., Montero, M. F., Mourino-Carballido, B., Nagata, T., Osma, N., Panton, A., Regaudie de Gioux, A., Reinthaler, T., Serret, P., Sulpis, O., Uchimiya, Mario., Wang, B., Wang, Q., & Yokokawa, T. (2026). A global dataset of marine pelagic microbial respiration. NERC EDS British Oceanographic Data Centre NOC. https://www.bodc.ac.uk/data/published_data_library/catalogue/10.5285/4b2a5ac6-b6db-c98e-e063-7086abc040c6 </ref>		<ref name="Robinson2026">Robinson, C., Seguro, I., Dall'Olmo, G., Moncoiffe, G., Aranguren-Gassis, M., Aristegui, J., Azzaro, M., Baek, Y. J., Baltar, F., Cohn, M. R., Eissler, Y., Evans, C., Fennel, K., Fernandez-Urruzola, I., Ferron, S., Fukuda, H., Garcia-Martin, E. E., Gifford, S., Goddard-Dwyer, M., Hernandez-Hernandez, N., Herndl, Y., Hyun, J. H., Kim, B., Kirchman, D., Kitidis, Vassilis., LaBrie, R., Lefevre, D., Lonborg, C., Maranger, R., Martinez-Garcia, S., Montero, M. F., Mourino-Carballido, B., Nagata, T., Osma, N., Panton, A., Regaudie de Gioux, A., Reinthaler, T., Serret, P., Sulpis, O., Uchimiya, Mario., Wang, B., Wang, Q., & Yokokawa, T. (2026). A global dataset of marine pelagic microbial respiration. NERC EDS British Oceanographic Data Centre NOC. https://www.bodc.ac.uk/data/published_data_library/catalogue/10.5285/4b2a5ac6-b6db-c98e-e063-7086abc040c6 </ref>

Anabelvonjackowski: /* References */

2026-05-15T17:54:45Z

References

← Older revision		Revision as of 10:54, 15 May 2026
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	<ref name="Robinson2005">Robinson, C., & Williams, P. J. l. B. (2005). Respiration and its measurement in surface marine waters. In: del Giorgio, P. A., & Williams, P. J. l. B. (Eds.), ''Respiration in Aquatic Ecosystems''. Oxford University Press, Oxford, pp. 147–180. https://doi.org</ref>		<ref name="Robinson2005">Robinson, C., & Williams, P. J. l. B. (2005). Respiration and its measurement in surface marine waters. In: del Giorgio, P. A., & Williams, P. J. l. B. (Eds.), ''Respiration in Aquatic Ecosystems''. Oxford University Press, Oxford, pp. 147–180. https://doi.org</ref>

	<ref name="Robinson2026">Robinson, C., Seguro, I., Dall'Olmo, G., Moncoiffe, G., Aranguren-Gassis, M., Aristegui, J., Azzaro, M., Baek, Y. J., Baltar, F., Cohn, M. R., Eissler, Y., Evans, C., Fennel, K., Fernandez-Urruzola, I., Ferron, S., Fukuda, H., Garcia-Martin, E. E., Gifford, S., Goddard-Dwyer, M., Hernandez-Hernandez, N., Herndl, Y., Hyun, J. H., Kim, B., Kirchman, D., Kitidis, Vassilis., LaBrie, R., Lefevre, D., Lonborg, C., Maranger, R., Martinez-Garcia, S., Montero, M. F., Mourino-Carballido, B., Nagata, T., Osma, N., Panton, A., Regaudie de Gioux, A., Reinthaler, T., Serret, P., Sulpis, O., Uchimiya, Mario., Wang, B., Wang, Q., & Yokokawa, T. (2026). A global dataset of marine pelagic microbial respiration. NERC EDS British Oceanographic Data Centre NOC. https://~~doi~~.~~org~~</ref>		<ref name="Robinson2026">Robinson, C., Seguro, I., Dall'Olmo, G., Moncoiffe, G., Aranguren-Gassis, M., Aristegui, J., Azzaro, M., Baek, Y. J., Baltar, F., Cohn, M. R., Eissler, Y., Evans, C., Fennel, K., Fernandez-Urruzola, I., Ferron, S., Fukuda, H., Garcia-Martin, E. E., Gifford, S., Goddard-Dwyer, M., Hernandez-Hernandez, N., Herndl, Y., Hyun, J. H., Kim, B., Kirchman, D., Kitidis, Vassilis., LaBrie, R., Lefevre, D., Lonborg, C., Maranger, R., Martinez-Garcia, S., Montero, M. F., Mourino-Carballido, B., Nagata, T., Osma, N., Panton, A., Regaudie de Gioux, A., Reinthaler, T., Serret, P., Sulpis, O., Uchimiya, Mario., Wang, B., Wang, Q., & Yokokawa, T. (2026). A global dataset of marine pelagic microbial respiration. NERC EDS British Oceanographic Data Centre NOC. https://www.bodc.ac.uk/data/published_data_library/catalogue/10.5285/4b2a5ac6-b6db-c98e-e063-7086abc040c6 </ref>

	<ref name="RegaudiedeGioux2010">Regaudie-de-Gioux, A., & Duarte, C. M. (2010). Plankton metabolism in the Greenland Sea during the polar summer of 2007. ''Polar Biology'', 33, 1651–1660. https://doi.org/10.1007/s00300-010-0792-1</ref>		<ref name="RegaudiedeGioux2010">Regaudie-de-Gioux, A., & Duarte, C. M. (2010). Plankton metabolism in the Greenland Sea during the polar summer of 2007. ''Polar Biology'', 33, 1651–1660. https://doi.org/10.1007/s00300-010-0792-1</ref>

Anabelvonjackowski: /* References */

2026-05-15T17:53:50Z

References

← Older revision		Revision as of 10:53, 15 May 2026
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	== References ==		== References ==
	<references>		<references>
	<ref name="Kirchman2009">Kirchman, D.L., Hill, V., Cottrell, M.T., Gradinger, R., Malmstrom, R.R., Parker, A., 2009a. Standing stocks, production, and respiration of phytoplankton and heterotrophic bacteria in the western Arctic Ocean. ''Deep Sea Research Part II: Topical Studies in Oceanography'', 56(15), 1237–1248. https://doi.org/10.1016/j.dsr2.2008.10.018</ref>		<ref name="Kirchman2009">Kirchman, D. L., Hill, V., Cottrell, M. T., Gradinger, R., Malmstrom, R. R., & Parker, A. (2009a). Standing stocks, production, and respiration of phytoplankton and heterotrophic bacteria in the western Arctic Ocean. ''Deep Sea Research Part II: Topical Studies in Oceanography'', 56(15), 1237–1248. https://doi.org/10.1016/j.dsr2.2008.10.018</ref>

	<ref name="Meon2004">Meon, B., Amon, R., 2004. Heterotrophic bacterial activity and fluxes of dissolved free amino		<ref name="Meon2004">Meon, B., & Amon, R. (2004). Heterotrophic bacterial activity and fluxes of dissolved free amino acids and glucose in the Arctic rivers Ob, Yenisei and the adjacent Kara Sea. ''Aquatic Microbial Ecology'', 37, 121–135. https://doi.org/10.3354/ame037121</ref>
	acids and glucose in the Arctic rivers Ob, Yenisei and the adjacent Kara Sea. ~~Aquat. Microb. Ecol.~~ 37, 121–135.https://doi.org/10.3354/ame037121</ref>

			<ref name="Ortega-Retuerta2012">Ortega-Retuerta, E., Jeffrey, W. H., Babin, M., Bélanger, S., Benner, R., Marie, D., Matsuoka, A., Raimbault, P., & Joux, F. (2012). Carbon fluxes in the Canadian Arctic: patterns and drivers of bacterial abundance, production and respiration on the Beaufort Sea margin. ''Biogeosciences'', 9(9), 3679–3692. https://doi.org/10.5194/bg-9-3679-2012</ref>

			<ref name="Robinson2008">Robinson, C. (2008). Heterotrophic Bacterial Respiration. In: Kirchman, D. L. (Ed.), ''Microbial Ecology of the Oceans''. Wiley-Blackwell, New Jersey, pp. 299–334.</ref>

	<ref name="~~Ortega-Retuerta2012~~">~~Ortega-Retuerta~~, E., ~~Jeffrey~~, W. H.~~, Babin, M~~.~~, Bélanger, S~~.~~, Benner, R~~., ~~Marie, D~~.~~, Matsuoka,~~ A., ~~Raimbault~~, P.~~, & Joux, F~~. (~~2012~~)~~. Carbon fluxes in the Canadian Arctic: patterns and drivers of bacterial abundance~~, ~~production and respiration on the Beaufort Sea margin.~~ ''~~Biogeosciences~~'', ~~9(9)~~, ~~3679–3692~~. https://doi.org~~/10.5194/bg-9-3679-2012~~</ref>		<ref name="Robinson2005">Robinson, C., & Williams, P. J. l. B. (2005). Respiration and its measurement in surface marine waters. In: del Giorgio, P. A., & Williams, P. J. l. B. (Eds.), ''Respiration in Aquatic Ecosystems''. Oxford University Press, Oxford, pp. 147–180. https://doi.org</ref>

	<ref name="~~Robinson2005~~">Robinson, C., ~~2008~~. ~~Heterotrophic Bacterial Respiration~~, ~~in:~~ Kirchman, D.L. ~~(Ed~~.), ~~Microbial~~		<ref name="Robinson2026">Robinson, C., Seguro, I., Dall'Olmo, G., Moncoiffe, G., Aranguren-Gassis, M., Aristegui, J., Azzaro, M., Baek, Y. J., Baltar, F., Cohn, M. R., Eissler, Y., Evans, C., Fennel, K., Fernandez-Urruzola, I., Ferron, S., Fukuda, H., Garcia-Martin, E. E., Gifford, S., Goddard-Dwyer, M., Hernandez-Hernandez, N., Herndl, Y., Hyun, J. H., Kim, B., Kirchman, D., Kitidis, Vassilis., LaBrie, R., Lefevre, D., Lonborg, C., Maranger, R., Martinez-Garcia, S., Montero, M. F., Mourino-Carballido, B., Nagata, T., Osma, N., Panton, A., Regaudie de Gioux, A., Reinthaler, T., Serret, P., Sulpis, O., Uchimiya, Mario., Wang, B., Wang, Q., & Yokokawa, T. (2026). A global dataset of marine pelagic microbial respiration. NERC EDS British Oceanographic Data Centre NOC. https://doi.org</ref>
	~~Ecology of the Oceans~~. ~~Wiley~~-~~Blackwell~~, ~~New Jersey~~, pp. ~~299–334~~.</ref>

	<ref name="~~Robinson2008~~">~~Carol Robinson and Peter J~~. ~~le B~~. ~~Williams~~ (~~2005~~) ~~Respiration and its measurement~~ in ~~surface marine waters~~. ~~doi~~: ~~10.1093~~/~~acprof:oso~~/~~9780198527084~~.~~003~~.~~0009 In book: Respiration in Aquatic Ecosystems~~ </ref>		<ref name="RegaudiedeGioux2010">Regaudie-de-Gioux, A., & Duarte, C. M. (2010). Plankton metabolism in the Greenland Sea during the polar summer of 2007. ''Polar Biology'', 33, 1651–1660. https://doi.org/10.1007/s00300-010-0792-1</ref>

	<ref name="~~RegaudiedeGioux2010~~">~~Regaudie-de-Gioux~~, A., ~~Duarte~~, C.M., ~~2010~~. ~~Plankton metabolism in the Greenland~~ Sea ~~during the polar summer of 2007. Polar Biol 33~~, ~~1651–1660~~. https://doi.org/10.~~1007~~/~~s00300-010-0792-1~~ </ref>		<ref name="Williams2004">Williams, P. J. M., & Karl, D. M. (2004). Net community production and metabolic balance at the oligotrophic ocean site, station ALOHA. ''Deep Sea Research Part I: Oceanographic Research Papers'', 51(11), 1563–1578. https://doi.org/10.1016/j.dsr.2004.07.001</ref>

	<ref name="Williams2004">Peter J. le B. Williams, Paul J. Morris, David M. Karl, Net community production and metabolic balance at the oligotrophic ocean site, station ALOHA, Deep Sea Research Part I: Oceanographic Research Papers,Volume 51, Issue 11,2004,https://doi.org/10.1016/j.dsr.2004.07.001 </ref>

Anabelvonjackowski: /* Bacterial Respiration */

2026-05-15T17:52:31Z

Bacterial Respiration

← Older revision		Revision as of 10:52, 15 May 2026
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	\| Arctic Ocean \|\| Kara Sea \|\| Aug-Sep 2001 \|\| 0.48 ± 0.57 \|\| Meon and Amon (2004) <ref name="Meon2004" />		\| Arctic Ocean \|\| Kara Sea \|\| Aug-Sep 2001 \|\| 0.48 ± 0.57 \|\| Meon and Amon (2004) <ref name="Meon2004" />
	\|}		\|}


			More CR and BR rates are available via the "A global dataset of marine pelagic microbial respiration" database <ref name="Robinson2026" />

	== References ==		== References ==

Anabelvonjackowski: /* References */

2026-05-15T17:41:09Z

References

← Older revision		Revision as of 10:41, 15 May 2026
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	<ref name="Ortega-Retuerta2012">Ortega-Retuerta, E., Jeffrey, W. H., Babin, M., Bélanger, S., Benner, R., Marie, D., Matsuoka, A., Raimbault, P., & Joux, F. (2012). Carbon fluxes in the Canadian Arctic: patterns and drivers of bacterial abundance, production and respiration on the Beaufort Sea margin. ''Biogeosciences'', 9(9), 3679–3692. https://doi.org/10.5194/bg-9-3679-2012</ref>		<ref name="Ortega-Retuerta2012">Ortega-Retuerta, E., Jeffrey, W. H., Babin, M., Bélanger, S., Benner, R., Marie, D., Matsuoka, A., Raimbault, P., & Joux, F. (2012). Carbon fluxes in the Canadian Arctic: patterns and drivers of bacterial abundance, production and respiration on the Beaufort Sea margin. ''Biogeosciences'', 9(9), 3679–3692. https://doi.org/10.5194/bg-9-3679-2012</ref>

	<ref name="~~Robinson2008~~">Robinson, C., 2008. Heterotrophic Bacterial Respiration, in: Kirchman, D.L. (Ed.), Microbial		<ref name="Robinson2005">Robinson, C., 2008. Heterotrophic Bacterial Respiration, in: Kirchman, D.L. (Ed.), Microbial
	Ecology of the Oceans. Wiley-Blackwell, New Jersey, pp. 299–334.</ref>		Ecology of the Oceans. Wiley-Blackwell, New Jersey, pp. 299–334.</ref>

			<ref name="Robinson2008">Carol Robinson and Peter J. le B. Williams (2005) Respiration and its measurement in surface marine waters. doi: 10.1093/acprof:oso/9780198527084.003.0009 In book: Respiration in Aquatic Ecosystems </ref>

	<ref name="RegaudiedeGioux2010">Regaudie-de-Gioux, A., Duarte, C.M., 2010. Plankton metabolism in the Greenland Sea during the polar summer of 2007. Polar Biol 33, 1651–1660. https://doi.org/10.1007/s00300-010-0792-1 </ref>		<ref name="RegaudiedeGioux2010">Regaudie-de-Gioux, A., Duarte, C.M., 2010. Plankton metabolism in the Greenland Sea during the polar summer of 2007. Polar Biol 33, 1651–1660. https://doi.org/10.1007/s00300-010-0792-1 </ref>

Anabelvonjackowski: /* Community Respiration */

2026-05-15T17:39:18Z

Community Respiration

← Older revision		Revision as of 10:39, 15 May 2026
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	\|-		\|-
	\| Pacific Ocean \|\| Station ALOHA \|\| May 2001 - May 2002 \|\| 0-1.5 \|\| Williams et al., (2004) <ref name="Williams2004" />		\| Pacific Ocean \|\| Station ALOHA \|\| May 2001 - May 2002 \|\| 0-1.5 \|\| Williams et al., (2004) <ref name="Williams2004" />
			\|-
			\| Global Ocean \|\| Global \|\| - \|\| 3.3 ± 0.15 \|\| Robinson & Williams et al., (2005) <ref name="Robinson2005" />
	\|}		\|}

Anabelvonjackowski: /* References */

2026-05-15T17:32:38Z

References

← Older revision		Revision as of 10:32, 15 May 2026
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	<ref name="RegaudiedeGioux2010">Regaudie-de-Gioux, A., Duarte, C.M., 2010. Plankton metabolism in the Greenland Sea during the polar summer of 2007. Polar Biol 33, 1651–1660. https://doi.org/10.1007/s00300-010-0792-1 </ref>		<ref name="RegaudiedeGioux2010">Regaudie-de-Gioux, A., Duarte, C.M., 2010. Plankton metabolism in the Greenland Sea during the polar summer of 2007. Polar Biol 33, 1651–1660. https://doi.org/10.1007/s00300-010-0792-1 </ref>

			<ref name="Williams2004">Peter J. le B. Williams, Paul J. Morris, David M. Karl, Net community production and metabolic balance at the oligotrophic ocean site, station ALOHA, Deep Sea Research Part I: Oceanographic Research Papers,Volume 51, Issue 11,2004,https://doi.org/10.1016/j.dsr.2004.07.001 </ref>




	</references>		</references>

Anabelvonjackowski: /* Community Respiration */

2026-05-15T17:31:30Z

Community Respiration

← Older revision		Revision as of 10:31, 15 May 2026
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	\| Arctic Ocean \|\| Greenland Current \|\| July 2007 \|\| 2.1 ± 1.02 \|\| Regaudie-de-Gioux and Duarte (2010) <ref name="RegaudiedeGioux2010" />		\| Arctic Ocean \|\| Greenland Current \|\| July 2007 \|\| 2.1 ± 1.02 \|\| Regaudie-de-Gioux and Duarte (2010) <ref name="RegaudiedeGioux2010" />
			\|-
			\| Pacific Ocean \|\| Station ALOHA \|\| May 2001 - May 2002 \|\| 0-1.5 \|\| Williams et al., (2004) <ref name="Williams2004" />
	\|}		\|}