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* [[Page authors|Page authors]]: [[User:Kate Evans|Kate Evans]]
{{BreadcrumbsSecondaryProduction}}
* [[Responsible curator|Responsible curator]]:  [[User:Kate Evans|Kate Evans]]
 
* [[Page authors|Page authors]]: [[PRIMO]]
* [[Responsible curator|Responsible curator]]:  [[User:Hagi BucknWise|Hagen Buck-Wiese]]
----
----


__TOC__
__TOC__
<div class="model-box">
<div class="model-box">
{| class="model-ib"
{| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;"
! What is being measured in 1 - 3 words
! Polysaccharide hydrolysis
|-
|-
| '''Approach:''' tracer quantification, remote sensing, sequencing, etc.
| '''Approach:''' enzyme assay
|-
|-
| '''Context:''' ''in situ'', incubation
| '''Context:''' incubation, lab
|-
|-
| '''Spatial scale:''' mL, km<sup>2</sup>
| '''Spatial scale:''' low
|-
|-
| '''Temporal scale:''' seconds, days, seasons
| '''Temporal scale:''' low; hours to days
|-
|-
| '''Units:'''  
| '''Units:''' mol monomer L-1 h-1
|-
|-
| '''Community captured:''' all, 0.7 - 3 µm, target species
| '''Community captured:''' heterotrophs
|-
|-
| '''Co-measurements:''' Other measurements required for interpretation of results e.g., temperature, salinity, wind speed history
| '''Co-measurements:''' cell abundance
|}
|}
</div>
</div>
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== Method Overview ==
== Method Overview ==


== Output ==
This enzyme assay measures polysaccharide hydrolysis by time course incubations with fluorescently labeled substrate (fluoresceinamine labeled biopolymers)<ref name="Arnosti1996">Arnosti (1996). A new method for measuring polysaccharide hydrolysis rates in marine environments. ''Organic Geochemistry'', 25(1-2), 105-115. https://doi.org/10.1016/S0146-6380(96)00112-X</ref>.
 
The assay is performed in discrete mode on bulk, size-fract, single cell samples.


=== Scale of measurement ===
=== Scale of measurement ===
Typically performed on replicate samples collected at a sampling station at one point in time. Sampling effort is relatively high impairing resolution in space or time. Time course incubations last hours to days, further restricting temporal resolution.


=== Data generated ===
=== Data generated ===
These measurements can provide hydrolysis rates per unit time.


=== Units & currency ===
=== Units & currency ===
Units are mol monomer L-1 h-1. The currency is carbon.


=== Sample size ===
=== Sample size ===


=== Repositories & databases ===
Typical samples are < 1 L in volume.


e.g. [https://github.com/raw-lab/NFixDB nFixDB]
== Limitations ==


== Limitations ==
Rate measurements to estimate Vmax, yielding potential rates. Bottle effects are likely to occur.


== Example Applications & Protocols ==
== Example Applications & Protocols ==


=== Classic examples ===
=== Classic examples ===
* Classic/original papers
* Arnosti (1996) ''A new method for measuring polysaccharide hydrolysis rates in marine environments'' <ref name="Arnosti1996" />


=== Recent applications ===
=== Recent applications ===
* New papers + protocol links (e.g. word doc used in lab) if applicable
* Martinez-Garcia et al. (2012) ''Capturing Single Cell Genomes of Active Polysaccharide Degraders: An Unexpected Contribution of Verrucomicrobia'' <ref name="Martinez-Garcia2012">Martinez-Garcia et al. (2012). Capturing Single Cell Genomes of Active Polysaccharide Degraders: An Unexpected Contribution of Verrucomicrobia. ''PLoS ONE'', 7(4), e35314. https://doi.org/10.1371/journal.pone.0035314</ref>
 
=== Common calculations/conversions ===
* E.g. conversion to standard units


== References ==
== References ==


[[Category:Main Pages|Model types]]
[[Category:Main Pages|Model types]]

Latest revision as of 11:30, 11 May 2026

Template:BreadcrumbsSecondaryProduction

Polysaccharide hydrolysis
Approach: enzyme assay
Context: incubation, lab
Spatial scale: low
Temporal scale: low; hours to days
Units: mol monomer L-1 h-1
Community captured: heterotrophs
Co-measurements: cell abundance

Method Overview

This enzyme assay measures polysaccharide hydrolysis by time course incubations with fluorescently labeled substrate (fluoresceinamine labeled biopolymers)[1].

The assay is performed in discrete mode on bulk, size-fract, single cell samples.

Scale of measurement

Typically performed on replicate samples collected at a sampling station at one point in time. Sampling effort is relatively high impairing resolution in space or time. Time course incubations last hours to days, further restricting temporal resolution.

Data generated

These measurements can provide hydrolysis rates per unit time.

Units & currency

Units are mol monomer L-1 h-1. The currency is carbon.

Sample size

Typical samples are < 1 L in volume.

Limitations

Rate measurements to estimate Vmax, yielding potential rates. Bottle effects are likely to occur.

Example Applications & Protocols

Classic examples

  • Arnosti (1996) A new method for measuring polysaccharide hydrolysis rates in marine environments [1]

Recent applications

  • Martinez-Garcia et al. (2012) Capturing Single Cell Genomes of Active Polysaccharide Degraders: An Unexpected Contribution of Verrucomicrobia [2]

References

  1. ↑ 1.0 1.1 Arnosti (1996). A new method for measuring polysaccharide hydrolysis rates in marine environments. Organic Geochemistry, 25(1-2), 105-115. https://doi.org/10.1016/S0146-6380(96)00112-X
  2. ↑ Martinez-Garcia et al. (2012). Capturing Single Cell Genomes of Active Polysaccharide Degraders: An Unexpected Contribution of Verrucomicrobia. PLoS ONE, 7(4), e35314. https://doi.org/10.1371/journal.pone.0035314
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