Enzyme assay with DNS: Difference between revisions
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== Method Overview == | == Method Overview == | ||
This enzyme assay measures polysaccharide hydrolysis by time course | This enzyme assay measures polysaccharide hydrolysis by time course incubation with DNS (2,4-dinitrophenylsulfonyl-group) <ref name="Kim2014">Kim et al. (2014). Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application. ''Journal of Microbiology and Biotechnology'', 24(11), 1559-1565. https://doi.org/10.4014/jmb.1405.05052</ref>. | ||
The assay is performed on bulk samples. | The assay is performed on bulk samples. | ||
Revision as of 11:38, 11 May 2026
Template:BreadcrumbsSecondaryProduction
| Polysaccharide hydrolysis |
|---|
| Approach: enzyme assay |
| Context: incubation, lab |
| Spatial scale: low |
| Temporal scale: low; hours to days |
| Units: mol mL-1 substrate h-1 |
| Community captured: heterotrophs |
| Co-measurements: cell abundance |
Method Overview
This enzyme assay measures polysaccharide hydrolysis by time course incubation with DNS (2,4-dinitrophenylsulfonyl-group) [1].
The assay is performed on bulk samples.
Scale of measurement
Data generated
Units & currency
Units are mol mL-1 substrate h-1. The currency is carbon.
Sample size
Typical samples are < 1 L in volume.
Repositories & databases
Limitations
Reports potential rates (Vmax) and bottle effects can occur during incubation period.
Example Applications & Protocols
Classic examples
- Kim et al. (2014) Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application [1]
Recent applications
Common calculations/conversions
References
- ↑ 1.0 1.1 Kim et al. (2014). Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application. Journal of Microbiology and Biotechnology, 24(11), 1559-1565. https://doi.org/10.4014/jmb.1405.05052