Enzyme assay with RBB labeled polysaccharides: Difference between revisions
Created page with "{{BreadcrumbsSecondaryProduction}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Polysaccharide hydrolysis |- | '''Approach:''' enzyme assay |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' low |- | '''Temporal scale:''' low; hours to days |- | '''Units:'''..." |
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== Method Overview == | == Method Overview == | ||
Time course incubation with RBB labeled polysaccharides<ref name=" | Time course incubation with RBB labeled polysaccharides<ref name="Wirth and Wolf">Wirth & Wolf (1992). Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons. ''Soil Biology and Biochemistry'', 24(6), 511-519. https://doi.org/10.1016/0038-0717(92)90074-8</ref>. | ||
Applicable variants include: bulk. | Applicable variants include: bulk. | ||
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=== Classic examples === | === Classic examples === | ||
* Wirth & Wolf (1992) ''Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons'' <ref name=" | * Wirth & Wolf (1992) ''Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons'' <ref name="Wirth and Wolf" /> | ||
=== Recent applications === | === Recent applications === | ||
* Zohary et al. (2000) ''The role of nutrients in decomposition of a thecate dinoflagellate'' <ref name=" | * Zohary et al. (2000) ''The role of nutrients in decomposition of a thecate dinoflagellate'' <ref name="Zohary et al">Zohary et al. (2000). The role of nutrients in decomposition of a thecate dinoflagellate. ''Limnology and Oceanography'', 45(1), 123-130. https://doi.org/10.4319/lo.2000.45.1.0123</ref> | ||
=== Common calculations/conversions === | === Common calculations/conversions === | ||
Revision as of 10:53, 11 May 2026
Template:BreadcrumbsSecondaryProduction
| Polysaccharide hydrolysis |
|---|
| Approach: enzyme assay |
| Context: incubation, lab |
| Spatial scale: low |
| Temporal scale: low; hours to days |
| Units: relative OD units |
| Community captured: heterotrophs |
| Co-measurements: cell abundance |
Method Overview
Time course incubation with RBB labeled polysaccharides[1].
Applicable variants include: bulk.
Scale of measurement
Spatial resolution is low (horizontal) and low (vertical). Temporal resolution is low, with a time integration of hours to days.
Data generated
Yields rates in units of relative OD units.
Units & currency
Units are relative OD units. The currency is carbon.
Sample size
< L.
Repositories & databases
Limitations
rate measurements at max (Vmax). bottle effects, potential rates.
Example Applications & Protocols
Classic examples
- Wirth & Wolf (1992) Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons [1]
Recent applications
- Zohary et al. (2000) The role of nutrients in decomposition of a thecate dinoflagellate [2]
Common calculations/conversions
References
- ↑ 1.0 1.1 Wirth & Wolf (1992). Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons. Soil Biology and Biochemistry, 24(6), 511-519. https://doi.org/10.1016/0038-0717(92)90074-8
- ↑ Zohary et al. (2000). The role of nutrients in decomposition of a thecate dinoflagellate. Limnology and Oceanography, 45(1), 123-130. https://doi.org/10.4319/lo.2000.45.1.0123