Enzyme assay with RBB labeled polysaccharides
Template:BreadcrumbsSecondaryProduction
| Polysaccharide hydrolysis |
|---|
| Approach: enzyme assay |
| Context: incubation, lab |
| Spatial scale: low |
| Temporal scale: low; hours to days |
| Units: relative OD units |
| Community captured: heterotrophs |
| Co-measurements: cell abundance |
Method Overview
This enzyme assay measures polysaccharide hydrolysis by time course incubation with RBB labeled polysaccharides[1].
The assay is performed in discrete mode on bulk samples.
Scale of measurement
Data generated
Units & currency
Units are relative OD units. The currency is carbon.
Sample size
Typical samples are < 1 L in volume.
Repositories & databases
Limitations
rate measurements at max (Vmax). bottle effects, potential rates.
Example Applications & Protocols
Classic examples
- Wirth & Wolf (1992) Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons [1]
Recent applications
- Zohary et al. (2000) The role of nutrients in decomposition of a thecate dinoflagellate [2]
Common calculations/conversions
References
- ↑ 1.0 1.1 Wirth & Wolf (1992). Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons. Soil Biology and Biochemistry, 24(6), 511-519. https://doi.org/10.1016/0038-0717(92)90074-8
- ↑ Zohary et al. (2000). The role of nutrients in decomposition of a thecate dinoflagellate. Limnology and Oceanography, 45(1), 123-130. https://doi.org/10.4319/lo.2000.45.1.0123