Enzyme assay with RBB labeled polysaccharides
Template:BreadcrumbsSecondaryProduction
| Polysaccharide hydrolysis |
|---|
| Approach: enzyme assay |
| Context: incubation, lab |
| Spatial scale: low |
| Temporal scale: low; hours to days |
| Units: relative OD units |
| Community captured: heterotrophs |
| Co-measurements: cell abundance |
Method Overview
Time course incubation with RBB labeled polysaccharidesCite error: Invalid <ref> tag; name cannot be a simple integer. Use a descriptive title.
Applicable variants include: bulk.
Scale of measurement
Spatial resolution is low (horizontal) and low (vertical). Temporal resolution is low, with a time integration of hours to days.
Data generated
Yields rates in units of relative OD units.
Units & currency
Units are relative OD units. The currency is carbon.
Sample size
< L.
Repositories & databases
Limitations
rate measurements at max (Vmax). bottle effects, potential rates.
Example Applications & Protocols
Classic examples
- Wirth & Wolf (1992) Micro-plate colourimetric assay for Endo -acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons Cite error: Invalid
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Recent applications
- Zohary et al. (2000) The role of nutrients in decomposition of a thecate dinoflagellate [1]
Common calculations/conversions
References
- ↑ Zohary et al. (2000). The role of nutrients in decomposition of a thecate dinoflagellate. Limnology and Oceanography, 45(1), 123-130. https://doi.org/10.4319/lo.2000.45.1.0123