High-throughput sequencing of RNA transcribed by an individual or population of a given organism, or a community of species.
| Approach: filtering, RNA extraction, sequencing, bioinformatics
|
| Context: in situ, culturing, incubations
|
| Spatial scale: L
|
| Temporal scale: seconds, days, seasons
|
| Units: gene expression, e.g. TPM
|
| Community captured: size-fractioned, e.g. 0.2 - 250 µm, cultured or target species
|
| Co-measurements: Other measurements required for interpretation of results e.g., temperature, salinity, nutrients, physiological data
|
Method Overview
Output
Scale of measurement
Data generated
Units & currency
Sample size
Repositories & databases
Limitations
Example Applications & Protocols
Classic examples
Recent applications
Common calculations/conversions
- Normalised expression, for example transcripts per million (TPM)
- Quantitative estimates, for example transcripts per L (TPL)
References
