Enzyme assay/alkaline phosphatase with fluorescently labeled substrate
Template:BreadcrumbsSecondaryProduction
| Alkaline phosphatase |
|---|
| Approach: enzyme assay |
| Context: incubation, lab |
| Spatial scale: low |
| Temporal scale: low; hours to days |
| Units: mol fl tag L-1 h-1 |
| Community captured: microalgae and heterotrophs |
| Co-measurements: cell abundance |
Method Overview
This enzyme assay measures alkaline phosphatase by time course incubations with fluorescently labeled substrate.
The assay is performed in continous mode (flow through fluorimeter) mode on bulk, size-fractionated, or single cell samples.
Scale of measurement
Data generated
Units & currency
Units are mol fl tag L-1 h-1. The currency is P.
Sample size
Typical samples are < 1 L in volume.
Repositories & databases
Limitations
Example Applications & Protocols
Classic examples
Recent applications
- Su et al. (2023) A dataset of global ocean alkaline phosphatase activity [1]
- Thomson et al. (2020) Relative Importance of Phosphodiesterase vs. Phosphomonoesterase (Alkaline Phosphatase) Activities for Dissolved Organic Phosphorus Hydrolysis in Epi- and Mesopelagic Waters [2]
Common calculations/conversions
References
- ↑ Su et al. (2023). A dataset of global ocean alkaline phosphatase activity. Scientific Data, 10(1). https://doi.org/10.1038/s41597-023-02081-7
- ↑ Thomson et al. (2020). Relative Importance of Phosphodiesterase vs. Phosphomonoesterase (Alkaline Phosphatase) Activities for Dissolved Organic Phosphorus Hydrolysis in Epi- and Mesopelagic Waters. Frontiers in Earth Science, 8. https://doi.org/10.3389/feart.2020.560893