User contributions for Hagi BucknWise

06:3006:30, 24 September 2026 diff hist −16 Proteome Allocation Models No edit summary current
06:2906:29, 24 September 2026 diff hist +69 Proteome Allocation Models No edit summary Tag: Visual edit: Switched
06:0606:06, 24 September 2026 diff hist −1 Phosphorus →Degradation of dissolved organic phosphorous current Tag: Visual edit
06:0606:06, 24 September 2026 diff hist −1 Welcome to OceanWiki →Nutrient Fluxes current
06:0206:02, 24 September 2026 diff hist +1 Welcome to OceanWiki →Nutrient Fluxes Tag: Visual edit: Switched
05:5105:51, 24 September 2026 diff hist +27 Welcome to OceanWiki →Model Types Tag: Visual edit
05:4205:42, 24 September 2026 diff hist +26 Model Types →Cellular scale current
05:4005:40, 24 September 2026 diff hist +122 13-Carbon uptake →Example Applications & Protocols current Tag: Visual edit

21:2921:29, 4 June 2026 diff hist −179 Welcome to OceanWiki No edit summary

15:0215:02, 27 May 2026 diff hist +64 Model Types →Biome scale
14:5314:53, 27 May 2026 diff hist −49 Model Types No edit summary
14:3214:32, 27 May 2026 diff hist 0 Data Types No edit summary
14:2814:28, 27 May 2026 diff hist 0 Data Types No edit summary
14:2114:21, 27 May 2026 diff hist −457 Data Types No edit summary

19:0219:02, 13 May 2026 diff hist +3,283 N Fluorescent in situ hybridization microscope counts Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Life cycle stage abundance (FISH) |- | '''Approach:''' fluorescence in situ hybridisation (FISH) with life-stage-specific or species-specific probes |- | '''Context:''' ''in situ'', lab |- | '''Spa..." current
18:5918:59, 13 May 2026 diff hist +4,171 N Light or electron microscopic counts of life cycle stages/transitions Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Life cycle stage abundance (microscopy) |- | '''Approach:''' light or electron microscopy counts of morphologically distinct life stages |- | '''Context:''' ''in situ'', lab |- | '''Spatial scale:'..." current
18:5718:57, 13 May 2026 diff hist +3,277 N Size-fractionated extract spiking Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Allelopathy (size-fractionated extract spiking) |- | '''Approach:''' size-fractionated culture extracts added to target species; growth or Fv/Fm response measured |- | '''Context:''' lab |- | '''Sp..."
18:5518:55, 13 May 2026 diff hist +3,796 N Filtrate cross-culturing Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Allelopathy (filtrate cross-culturing) |- | '''Approach:''' cell-free filtrate of candidate allelopathic species added to target species culture; growth or motility response measured |- | '''Contex..." current
18:5318:53, 13 May 2026 diff hist +3,738 N Single-cell RNA-sequencing (community) Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Viral infection across the plankton community (scRNA-seq) |- | '''Approach:''' community-level single-cell RNA sequencing; viral transcripts detected in individually barcoded cells |- | '''Context:..." current
18:5018:50, 13 May 2026 diff hist +3,835 N Single-cell RNA-sequencing (population) Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Viral infection in individual cells (scRNA-seq, population) |- | '''Approach:''' single-cell RNA sequencing of sorted populations; viral transcript detection per cell |- | '''Context:''' ''in situ'..." current
18:4718:47, 13 May 2026 diff hist +3,288 N Bulk RNA marker-gene PCR analysis Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Viral infection marker gene expression (RT-qPCR) |- | '''Approach:''' RT-qPCR of virus-specific marker gene transcripts |- | '''Context:''' ''in situ'', lab |- | '''Spatial scale:''' point sample |..." current
18:1318:13, 13 May 2026 diff hist +3,869 N Dilution incubation experiments Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Viral lysis rate (virus dilution method) |- | '''Approach:''' dilution of viruses with 0.2 µm filtered seawater; host abundance tracked by FCM |- | '''Context:''' incubation |- | '''Spatial scale:..." current
18:0918:09, 13 May 2026 diff hist +4,199 N Quantification of virus particles Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Virus particle abundance |- | '''Approach:''' epifluorescence microscopy, flow cytometry, plaque assay, or MPN |- | '''Context:''' ''in situ'', incubation, lab |- | '''Spatial scale:''' point sampl..."
18:0718:07, 13 May 2026 diff hist +3,429 N Electron microscopy of cells Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Viral infection prevalence (TEM) |- | '''Approach:''' transmission electron microscopy (TEM); scoring intracellular virions |- | '''Context:''' ''in situ'', lab |- | '''Spatial scale:''' point samp..." current
18:0318:03, 13 May 2026 diff hist +3,214 N BrdU-labeled prey incorporation into things with chloroplasts Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Constitutive mixotrophy (BrdU-labelled prey) |- | '''Approach:''' BrdU-labelled bacteria ingested by pigmented cells; immunofluorescence detection |- | '''Context:''' field, lab |- | '''Spatial sca..." current
18:0118:01, 13 May 2026 diff hist +3,619 N Bead consumption rates by cells with chloroplasts Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Phagotrophic mixotrophy (bead ingestion by pigmented cells) |- | '''Approach:''' fluorescent bead ingestion by FCM-identified autofluorescent cells; epifluorescence microscopy |- | '''Context:''' f..." current
17:5917:59, 13 May 2026 diff hist +3,695 N Isotope (15N/13C) labeling and assimilation of organic matter Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Osmotrophic mixotrophy (<sup>13</sup>C/<sup>15</sup>N DOM assimilation) |- | '''Approach:''' <sup>13</sup>C/<sup>15</sup>N-labelled dissolved or particulate organic matter; EA-IRMS or nanoSIMS |- |..."
17:5417:54, 13 May 2026 diff hist +3,735 N LysoTracker Green Incorporation Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Phagotrophic mixotroph detection (LysoTracker Green) |- | '''Approach:''' LysoTracker Green staining of food vacuoles; comparison to autofluorescence by FCM or imaging FCM |- | '''Context:''' field..." current
17:5117:51, 13 May 2026 diff hist +4,411 N Stable isotope-labelled prey Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Bacterivory and prey identity (stable isotope probing) |- | '''Approach:''' RNA/DNA-SIP with <sup>13</sup>C/<sup>15</sup>N-labelled prey; ultracentrifugation and amplicon sequencing of heavy fracti..."
17:4917:49, 13 May 2026 diff hist +3,194 N Pulse-chase labeling of bacterial prey Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Bacterivory by mixotrophs (pulse-chase <sup>14</sup>C) |- | '''Approach:''' <sup>14</sup>C pulse labelling of bacterial prey; radioactivity in FCM-sorted pigmented cells |- | '''Context:''' incubat..." current
17:4717:47, 13 May 2026 diff hist +3,306 N Radioactively labeled prey surrogates Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Bacterivory (radiolabelled prey surrogates) |- | '''Approach:''' <sup>3</sup>H or <sup>14</sup>C radiolabelled bacteria; filtration and scintillation counting |- | '''Context:''' incubation |- | ''..." current
17:4117:41, 13 May 2026 diff hist +3,678 N Fluorescently labeled prey surrogates Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Bacterivory (fluorescently labelled prey surrogates) |- | '''Approach:''' fluorescently labelled bacteria (FLB) or beads added to seawater; ingestion counted by epifluorescence microscopy or FCM |-..." current
17:3917:39, 13 May 2026 diff hist +4,022 N Gut-fluorescence Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Mesozooplankton ingestion (gut fluorescence) |- | '''Approach:''' fluorometric measurement of gut pigment content × gut evacuation rate |- | '''Context:''' ''in situ'', lab |- | '''Spatial scale:'..." current
17:3717:37, 13 May 2026 diff hist +2,295 N Cell abundance Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Phytoplankton net growth rate (cell counting) |- | '''Approach:''' FCM or microscopy cell counts in time-course incubations |- | '''Context:''' incubation |- | '''Spatial scale:''' point sample |-..." current
17:3617:36, 13 May 2026 diff hist +3,588 N Size-fractionated incubation dilution experiments Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Microzooplankton grazing, size-fractionated |- | '''Approach:''' dilution method with FCM or HPLC tracking of size-class phytoplankton |- | '''Context:''' incubation |- | '''Spatial scale:''' point..." current
17:3417:34, 13 May 2026 diff hist +18 Grazing No edit summary current
17:3317:33, 13 May 2026 diff hist +5,310 N Incubation dilution experiments Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Microzooplankton grazing (dilution method) |- | '''Approach:''' dilution of whole seawater with 0.2 µm filtered seawater; Chl-a or FCM tracking |- | '''Context:''' incubation |- | '''Spatial scale..." current

17:2817:28, 12 May 2026 diff hist +4,542 N Calcification (13-Carbon uptake) Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Calcification rate (<sup>13</sup>C) |- | '''Approach:''' stable isotope (<sup>13</sup>C-bicarbonate) incubation; separation of PIC and POC fractions |- | '''Context:''' incubation, lab |- | '''Spatial..." current
17:2617:26, 12 May 2026 diff hist +3,401 N Biogenic silica accumulation Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Biogenic silica accumulation (light–dark bottles) |- | '''Approach:''' change in BSi concentration in light vs. dark incubation bottles |- | '''Context:''' incubation, ''in situ'' and lab |- | '''Sp..." current
17:2517:25, 12 May 2026 diff hist +4,789 N Silica production - PDMPO Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Biogenic silica production (PDMPO) |- | '''Approach:''' fluorescent silica dye (PDMPO) incorporation |- | '''Context:''' incubation, ''in situ'' and lab |- | '''Spatial scale:''' point sample; single..." current
17:2317:23, 12 May 2026 diff hist +3,787 N Silica production Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Biogenic silica production (<sup>32</sup>Si) |- | '''Approach:''' radiotracer (<sup>32</sup>Si) incubation; gross biogenic silica production |- | '''Context:''' incubation, ''in situ'' and lab |- | ''..." current
17:2117:21, 12 May 2026 diff hist +4,111 N Kinetics of silicon uptake Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Silicon uptake kinetics (V<sub>max</sub>, K<sub>s</sub>) |- | '''Approach:''' <sup>32</sup>Si incubations at multiple ambient silicic acid concentrations; Michaelis–Menten fitting |- | '''Context:''..." current
17:1917:19, 12 May 2026 diff hist +4,739 N Silicon uptake Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Silicon uptake (<sup>32</sup>Si) |- | '''Approach:''' radiotracer (<sup>32</sup>Si) incubation |- | '''Context:''' incubation, ''in situ'' and lab |- | '''Spatial scale:''' point sample |- | '''Tempor..." current
17:1417:14, 12 May 2026 diff hist +3,562 N Cobalamin uptake with 57Cobalt-B12 Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Cobalamin uptake (<sup>57</sup>Co-B12) |- | '''Approach:''' radiotracer (<sup>57</sup>Co-labelled cobalamin) incubation |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' point sample |- |..." current
17:1317:13, 12 May 2026 diff hist +4,100 N 67-Copper (half-life 62 h; incubation) & 64-Cu (half-life 12.7 h; lab) uptake Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Copper uptake (<sup>67/64</sup>Cu) |- | '''Approach:''' radiotracer (<sup>67</sup>Cu for ''in situ''; <sup>64</sup>Cu for lab) incubation |- | '''Context:''' incubation, lab |- | '''Spatial scale:'''..." current
17:1017:10, 12 May 2026 diff hist +3,107 N 54-Manganese uptake Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Manganese uptake (<sup>54</sup>Mn) |- | '''Approach:''' radiotracer (<sup>54</sup>Mn) incubation |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' point sample |- | '''Temporal scale:''' 4..." current
17:0917:09, 12 May 2026 diff hist +3,708 N 55-Iron uptake Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Iron uptake (<sup>55</sup>Fe) |- | '''Approach:''' radiotracer (<sup>55</sup>Fe) incubation |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' point sample |- | '''Temporal scale:''' 4–24..."
17:0717:07, 12 May 2026 diff hist +3,424 N Rates of DMS and DMSP oxidation to DMSO Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! DMS and DMSP oxidation rates |- | '''Approach:''' deuterium-labelled DMS/DMSP tracers; D<sub>3</sub>-DMSO and D<sub>6</sub>-DMSO formation by GC-MS |- | '''Context:''' ''in situ'' incubations, lab |-..." current
17:0517:05, 12 May 2026 diff hist +3,354 N Rates of DMSO reduction to DMS Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! DMSO reduction rate |- | '''Approach:''' deuterium-labelled DMSO tracer; D<sub>6</sub>-DMS formation measured by GC-MS |- | '''Context:''' ''in situ'' incubations, lab |- | '''Spatial scale:''' point..." current
17:0317:03, 12 May 2026 diff hist +3,561 N DMS/P/O cycling Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! DMS/DMSP/DMSO cycling rates |- | '''Approach:''' isotope dilution with deuterated DMS/DMSP/DMSO standards; GC-MS detection |- | '''Context:''' ''in situ'' |- | '''Spatial scale:''' point sample |- | '..." current

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