User contributions for Hagi BucknWise

13 May 2026

• 19:0219:02, 13 May 2026 diff hist +3,283 N Fluorescent in situ hybridization microscope counts Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Life cycle stage abundance (FISH) |- | '''Approach:''' fluorescence in situ hybridisation (FISH) with life-stage-specific or species-specific probes |- | '''Context:''' ''in situ'', lab |- | '''Spa..." current
• 18:5918:59, 13 May 2026 diff hist +4,171 N Light or electron microscopic counts of life cycle stages/transitions Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Life cycle stage abundance (microscopy) |- | '''Approach:''' light or electron microscopy counts of morphologically distinct life stages |- | '''Context:''' ''in situ'', lab |- | '''Spatial scale:'..." current
• 18:5718:57, 13 May 2026 diff hist +3,277 N Size-fractionated extract spiking Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Allelopathy (size-fractionated extract spiking) |- | '''Approach:''' size-fractionated culture extracts added to target species; growth or Fv/Fm response measured |- | '''Context:''' lab |- | '''Sp..." current
• 18:5518:55, 13 May 2026 diff hist +3,796 N Filtrate cross-culturing Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Allelopathy (filtrate cross-culturing) |- | '''Approach:''' cell-free filtrate of candidate allelopathic species added to target species culture; growth or motility response measured |- | '''Contex..." current
• 18:5318:53, 13 May 2026 diff hist +3,738 N Single-cell RNA-sequencing (community) Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Viral infection across the plankton community (scRNA-seq) |- | '''Approach:''' community-level single-cell RNA sequencing; viral transcripts detected in individually barcoded cells |- | '''Context:..." current
• 18:5018:50, 13 May 2026 diff hist +3,835 N Single-cell RNA-sequencing (population) Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Viral infection in individual cells (scRNA-seq, population) |- | '''Approach:''' single-cell RNA sequencing of sorted populations; viral transcript detection per cell |- | '''Context:''' ''in situ'..." current
• 18:4718:47, 13 May 2026 diff hist +3,288 N Bulk RNA marker-gene PCR analysis Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Viral infection marker gene expression (RT-qPCR) |- | '''Approach:''' RT-qPCR of virus-specific marker gene transcripts |- | '''Context:''' ''in situ'', lab |- | '''Spatial scale:''' point sample |..." current
• 18:1318:13, 13 May 2026 diff hist +3,869 N Dilution incubation experiments Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Viral lysis rate (virus dilution method) |- | '''Approach:''' dilution of viruses with 0.2 µm filtered seawater; host abundance tracked by FCM |- | '''Context:''' incubation |- | '''Spatial scale:..." current
• 18:0918:09, 13 May 2026 diff hist +4,199 N Quantification of virus particles Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Virus particle abundance |- | '''Approach:''' epifluorescence microscopy, flow cytometry, plaque assay, or MPN |- | '''Context:''' ''in situ'', incubation, lab |- | '''Spatial scale:''' point sampl..." current
• 18:0718:07, 13 May 2026 diff hist +3,429 N Electron microscopy of cells Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Viral infection prevalence (TEM) |- | '''Approach:''' transmission electron microscopy (TEM); scoring intracellular virions |- | '''Context:''' ''in situ'', lab |- | '''Spatial scale:''' point samp..." current
• 18:0318:03, 13 May 2026 diff hist +3,214 N BrdU-labeled prey incorporation into things with chloroplasts Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Constitutive mixotrophy (BrdU-labelled prey) |- | '''Approach:''' BrdU-labelled bacteria ingested by pigmented cells; immunofluorescence detection |- | '''Context:''' field, lab |- | '''Spatial sca..." current
• 18:0118:01, 13 May 2026 diff hist +3,619 N Bead consumption rates by cells with chloroplasts Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Phagotrophic mixotrophy (bead ingestion by pigmented cells) |- | '''Approach:''' fluorescent bead ingestion by FCM-identified autofluorescent cells; epifluorescence microscopy |- | '''Context:''' f..." current
• 17:5917:59, 13 May 2026 diff hist +3,695 N 15N/13C-labelled DOM/cells Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Osmotrophic mixotrophy (¹³C/¹⁵N DOM assimilation) |- | '''Approach:''' ¹³C/¹⁵N-labelled dissolved or particulate organic matter; EA-IRMS or nanoSIMS |- |..." current
• 17:5417:54, 13 May 2026 diff hist +3,735 N LysoTracker Green Incorporation Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Phagotrophic mixotroph detection (LysoTracker Green) |- | '''Approach:''' LysoTracker Green staining of food vacuoles; comparison to autofluorescence by FCM or imaging FCM |- | '''Context:''' field..." current
• 17:5117:51, 13 May 2026 diff hist +4,411 N Stable isotope-labelled prey Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Bacterivory and prey identity (stable isotope probing) |- | '''Approach:''' RNA/DNA-SIP with ¹³C/¹⁵N-labelled prey; ultracentrifugation and amplicon sequencing of heavy fracti..." current
• 17:4917:49, 13 May 2026 diff hist +3,194 N Pulse-chase labeling of bacterial prey Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Bacterivory by mixotrophs (pulse-chase ¹⁴C) |- | '''Approach:''' ¹⁴C pulse labelling of bacterial prey; radioactivity in FCM-sorted pigmented cells |- | '''Context:''' incubat..." current
• 17:4717:47, 13 May 2026 diff hist +3,306 N Radioactively labeled prey surrogates Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Bacterivory (radiolabelled prey surrogates) |- | '''Approach:''' ³H or ¹⁴C radiolabelled bacteria; filtration and scintillation counting |- | '''Context:''' incubation |- | ''..." current
• 17:4117:41, 13 May 2026 diff hist +3,678 N Fluorescently labeled prey surrogates Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Bacterivory (fluorescently labelled prey surrogates) |- | '''Approach:''' fluorescently labelled bacteria (FLB) or beads added to seawater; ingestion counted by epifluorescence microscopy or FCM |-..." current
• 17:3917:39, 13 May 2026 diff hist +4,022 N Gut-fluorescence Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Mesozooplankton ingestion (gut fluorescence) |- | '''Approach:''' fluorometric measurement of gut pigment content × gut evacuation rate |- | '''Context:''' ''in situ'', lab |- | '''Spatial scale:'..." current
• 17:3717:37, 13 May 2026 diff hist +2,295 N Cell abundance Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Phytoplankton net growth rate (cell counting) |- | '''Approach:''' FCM or microscopy cell counts in time-course incubations |- | '''Context:''' incubation |- | '''Spatial scale:''' point sample |-..." current
• 17:3617:36, 13 May 2026 diff hist +3,588 N Size-fractionated incubation dilution experiments Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Microzooplankton grazing, size-fractionated |- | '''Approach:''' dilution method with FCM or HPLC tracking of size-class phytoplankton |- | '''Context:''' incubation |- | '''Spatial scale:''' point..." current
• 17:3417:34, 13 May 2026 diff hist +18 Grazing No edit summary current
• 17:3317:33, 13 May 2026 diff hist +5,310 N Incubation dilution experiments Created page with "{{BreadcrumbsInteractions}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Microzooplankton grazing (dilution method) |- | '''Approach:''' dilution of whole seawater with 0.2 µm filtered seawater; Chl-a or FCM tracking |- | '''Context:''' incubation |- | '''Spatial scale..." current

12 May 2026

• 17:2817:28, 12 May 2026 diff hist +4,542 N Calcification (13-Carbon uptake) Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Calcification rate (¹³C) |- | '''Approach:''' stable isotope (¹³C-bicarbonate) incubation; separation of PIC and POC fractions |- | '''Context:''' incubation, lab |- | '''Spatial..." current
• 17:2617:26, 12 May 2026 diff hist +3,401 N Biogenic silica accumulation Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Biogenic silica accumulation (light–dark bottles) |- | '''Approach:''' change in BSi concentration in light vs. dark incubation bottles |- | '''Context:''' incubation, ''in situ'' and lab |- | '''Sp..." current
• 17:2517:25, 12 May 2026 diff hist +4,789 N Silica production - PDMPO Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Biogenic silica production (PDMPO) |- | '''Approach:''' fluorescent silica dye (PDMPO) incorporation |- | '''Context:''' incubation, ''in situ'' and lab |- | '''Spatial scale:''' point sample; single..." current
• 17:2317:23, 12 May 2026 diff hist +3,787 N Silica production Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Biogenic silica production (³²Si) |- | '''Approach:''' radiotracer (³²Si) incubation; gross biogenic silica production |- | '''Context:''' incubation, ''in situ'' and lab |- | ''..." current
• 17:2117:21, 12 May 2026 diff hist +4,111 N Kinetics of silicon uptake Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Silicon uptake kinetics (V_max, K_s) |- | '''Approach:''' ³²Si incubations at multiple ambient silicic acid concentrations; Michaelis–Menten fitting |- | '''Context:''..." current
• 17:1917:19, 12 May 2026 diff hist +4,739 N Silicon uptake Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Silicon uptake (³²Si) |- | '''Approach:''' radiotracer (³²Si) incubation |- | '''Context:''' incubation, ''in situ'' and lab |- | '''Spatial scale:''' point sample |- | '''Tempor..." current
• 17:1417:14, 12 May 2026 diff hist +3,562 N Cobalamin uptake with 57Cobalt-B12 Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Cobalamin uptake (⁵⁷Co-B12) |- | '''Approach:''' radiotracer (⁵⁷Co-labelled cobalamin) incubation |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' point sample |- |..." current
• 17:1317:13, 12 May 2026 diff hist +4,100 N 67-Copper (half-life 62 h; incubation) & 64-Cu (half-life 12.7 h; lab) uptake Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Copper uptake (^67/64Cu) |- | '''Approach:''' radiotracer (⁶⁷Cu for ''in situ''; ⁶⁴Cu for lab) incubation |- | '''Context:''' incubation, lab |- | '''Spatial scale:'''..." current
• 17:1017:10, 12 May 2026 diff hist +3,107 N 54-Manganese uptake Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Manganese uptake (⁵⁴Mn) |- | '''Approach:''' radiotracer (⁵⁴Mn) incubation |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' point sample |- | '''Temporal scale:''' 4..." current
• 17:0917:09, 12 May 2026 diff hist +3,708 N 55-Iron uptake Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Iron uptake (⁵⁵Fe) |- | '''Approach:''' radiotracer (⁵⁵Fe) incubation |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' point sample |- | '''Temporal scale:''' 4–24..." current
• 17:0717:07, 12 May 2026 diff hist +3,424 N Rates of DMS and DMSP oxidation to DMSO Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! DMS and DMSP oxidation rates |- | '''Approach:''' deuterium-labelled DMS/DMSP tracers; D₃-DMSO and D₆-DMSO formation by GC-MS |- | '''Context:''' ''in situ'' incubations, lab |-..." current
• 17:0517:05, 12 May 2026 diff hist +3,354 N Rates of DMSO reduction to DMS Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! DMSO reduction rate |- | '''Approach:''' deuterium-labelled DMSO tracer; D₆-DMS formation measured by GC-MS |- | '''Context:''' ''in situ'' incubations, lab |- | '''Spatial scale:''' point..." current
• 17:0317:03, 12 May 2026 diff hist +3,561 N DMS/P/O cycling Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! DMS/DMSP/DMSO cycling rates |- | '''Approach:''' isotope dilution with deuterated DMS/DMSP/DMSO standards; GC-MS detection |- | '''Context:''' ''in situ'' |- | '''Spatial scale:''' point sample |- | '..." current
• 17:0117:01, 12 May 2026 diff hist +3,075 N 32-P incorporation Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Phosphate uptake (³²P) |- | '''Approach:''' radiotracer (³²P-phosphate) incubation |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' point sample |- | '''Temporal sca..." current
• 16:5916:59, 12 May 2026 diff hist +3,076 N Reduction of phosphite to phosphate for growth (ptxABCD) Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Phosphite utilization (ptxABCD) |- | '''Approach:''' culture growth assay with phosphite as sole P source |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' culture flask (point) |- | '''Te..." current
• 16:5716:57, 12 May 2026 diff hist +2,991 N Cleavage of phosphate from 5'-nucleotides: 5'NT/5PN activity Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! 5'-nucleotidase activity (5'NT) |- | '''Approach:''' radiolabelled ATP substrate assay |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' point sample |- | '''Temporal scale:''' hours to da..." current
• 16:5616:56, 12 May 2026 diff hist +3,011 N Degradation of dissolved organic phosphorus (phosphonates): C-P lyase activity (CLA) Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! C-P lyase activity (CLA) |- | '''Approach:''' dansyl-labelled phosphonate substrate + HPLC fluorescence detection |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' point sample |- | '''Tem..." current
• 16:5416:54, 12 May 2026 diff hist +3,101 N Degradation of dissolved organic phosphorus (P-diesters): Phosphodiesterase activity (PDE) Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Phosphodiesterase activity (PDE) |- | '''Approach:''' fluorescent bis-MUF-phosphate substrate assay |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' point sample |- | '''Temporal scale:''..." current
• 16:5216:52, 12 May 2026 diff hist +4,294 N Degradation of dissolved organic phosphorus (P-monoesters): Alkaline phosphatase activity (APA) Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Alkaline phosphatase activity (APA) |- | '''Approach:''' fluorescent MUF-phosphate substrate assay |- | '''Context:''' incubation, lab |- | '''Spatial scale:''' point sample |- | '''Temporal scale:'''..." current
• 16:4916:49, 12 May 2026 diff hist +3,104 N Degradation of dissolved organic nitrogen: Endopeptidase activity Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Endopeptidase activity |- | '''Approach:''' fluorescent substrate assay (MCA-oligopeptide) |- | '''Context:''' lab incubation |- | '''Spatial scale:''' point sample |- | '''Temporal scale:''' hours to..." current
• 16:4716:47, 12 May 2026 diff hist +3,864 N Degradation of dissolved organic nitrogen: Leucine-aminopeptidase activity Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Leucine-aminopeptidase activity |- | '''Approach:''' fluorescent substrate assay (MCA-Leu) |- | '''Context:''' lab incubation |- | '''Spatial scale:''' point sample |- | '''Temporal scale:''' hours to..." current
• 16:4216:42, 12 May 2026 diff hist +3,660 N 13C- and 15N-labeled algal exudates (isotope tracing & nanoSIMS) Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Bacterial DOM assimilation (algal exudate tracing, nanoSIMS) |- | '''Approach:''' ¹³C/¹⁵N-labelled algal exudates + nanoSIMS single-cell analysis |- | '''Context:''' lab incubati..." current
• 16:1816:18, 12 May 2026 diff hist +3,174 N FISH staining of anammox bacteria Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Anammox cell abundance (CARD-FISH) |- | '''Approach:''' catalyzed reporter deposition – fluorescence in situ hybridization (CARD-FISH) |- | '''Context:''' lab |- | '''Spatial scale:''' point sample..." current
• 16:1616:16, 12 May 2026 diff hist +2,818 N Functional gene quantification (hzs, hzo) Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Anammox marker genes (hzs, hzo) |- | '''Approach:''' qPCR quantification of anammox-specific functional genes |- | '''Context:''' lab, environmental collection |- | '''Spatial scale:''' point sample |..." current
• 16:1416:14, 12 May 2026 diff hist +4,123 N 15N tracers (15N labeled gases upon addition of 15NO3, 15NO2, or 15NH4) Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Anammox rate (¹⁵N isotope pairing) |- | '''Approach:''' ¹⁵N isotope pairing; ¹⁵N₂ production measured by MIMS |- | '''Context:''' incubation, lab |- | '''Sp..." current
• 16:1216:12, 12 May 2026 diff hist +4,147 N Isotopic measurements---15N-labeled ammonium (15NH4+) accumulation rate in 15NO3- added incubation Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! DNRA rate (¹⁵NH₄⁺ accumulation) |- | '''Approach:''' ¹⁵NO₃^- addition; ¹⁵NH₄⁺ accumulation measured by..." current
• 16:0916:09, 12 May 2026 diff hist +3,980 N Natural Abundances of 15N and 18O Created page with "{{BreadcrumbsNutrients}} * Page authors: PRIMO * Responsible curator: Hagen Buck-Wiese ---- __TOC__ <div class="model-box"> {| class="model-ib" style="float:right; margin-left:1em; margin-bottom:1em;" ! Denitrification isotope fingerprint (δ¹⁵N, δ¹⁸O-NO₃) |- | '''Approach:''' natural abundance IRMS of δ¹⁵N and δ¹⁸O in nitrate |- | '''Context:..." current