Nitrogen Fixation

• Bulk uptake ¹⁵N₂
• Single cell uptake (SIP-SIMS/nanoSIMS; CHIP-SIMS; CARD-FISH paired with nanoSIMS)
• Acetylene Reduction Assays (ARA)
• nifH detection/quantification (qPCR, RT-qPCR; nifH database)
• nifH amplicon sequence
• H₂ supersaturation
• Nitrogenase quantification
• Other diazotroph marker genes: nifD, nifK